Project
Proposal on The Antimicrobial and
Phytochemical Activities of the Oil Extract of Neem seed and its Efficacy in Repelling
Insects

 

 

PROPOSED
METHODOLOGY

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Material preparation

Neem seeds used in this study was obtained from Sokoto
state. Prior to use, the Neem seeds were repeatedly washed to remove impurities
and sun dried for 8 days. Then Neem seeds were ground.

Oil Extraction

·        
Neem seeds will be extracted using the soxhlet extractor

·        
A mixture of ethanol and hexane in the ratio
60:40 respectively will be used

·        
Neem seed will be extracted with ratio of neem
seed powder to solvent mixture 1:5.

·        
Extraction will be conducted at 5 temperatures
level (30, 35, 40, 45, and 50 degrees Celsius) for 4 hours each. (This is to
determine the variance of temperature on oil yield)

·        
The extracted mixture will be distilled at 80
degrees Celsius to remove until the neem oil is visible. (About 1-hour
distillation time)

Phytochemical
Screening

 Project
Proposal on The Antimicrobial and
Phytochemical Activities of the Oil Extract of Neem seed and its Efficacy in Repelling
Insects

 

 

PROPOSED
METHODOLOGY

Material preparation

Neem seeds used in this study was obtained from Sokoto
state. Prior to use, the Neem seeds were repeatedly washed to remove impurities
and sun dried for 8 days. Then Neem seeds were ground.

Oil Extraction

·        
Neem seeds will be extracted using the soxhlet extractor

·        
A mixture of ethanol and hexane in the ratio
60:40 respectively will be used

·        
Neem seed will be extracted with ratio of neem
seed powder to solvent mixture 1:5.

·        
Extraction will be conducted at 5 temperatures
level (30, 35, 40, 45, and 50 degrees Celsius) for 4 hours each. (This is to
determine the variance of temperature on oil yield)

·        
The extracted mixture will be distilled at 80
degrees Celsius to remove until the neem oil is visible. (About 1-hour
distillation time)

Phytochemical
Screening

 

 

 

 

 

 

 

 

 

 

 

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