The of oil palm leaf respectively. Rats in group

The toxicity of petroleum hydrocarbon across the
living systems is now a common knowledge among the scientific community. What
is lacking is a mini-scale antidote that can be adopted by the inhabitants of
crude oil producing areas of the world. This was the reason for this study. The
study is comprised forty eight female rats divided into six groups of eight
rats each. Rats in control group were fed with diet without any treatment while
rats in groups 2 and 3 were fed with diets treated with known amount of oil
palm leaf respectively. Rats in group 4 were fed with crude oil contaminated
diet. Rats in groups 5 and 6 were fed with contaminated diet mixed with known
amount of ground oil palm leaf. Biochemical and histological analysis were
carried out after three and six months respectively. The results show that
pretreatment of crude oil contaminated diet with oil palm leaf tend to restore values of lipid peroxidation, xanthine oxidase
activity, superoxide dismutase activity and catalase activity close to control
values. Histological examination indicates
protective effect of oil palm leaf against deleterious effect of crude oil on
the kidney.   Thus, it is pertinent to state that
there exist potentials in the use oil palm leaf in the treatment of crude oil
toxicity. And indeed setting a fresh agenda for further serious scientific




Keywords: Crude oil, Kidney, Lipid peroxidation, Oil
palm, .Xanthine oxidase,

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 1.0 Introduction

 Humans and animals get exposed to crude oil or
its byproducts when these chemicals are released into the surroundings during
oil exploration activities, equipment failures, corrosion, illegal bunkering,
usage, oil theft and illicit refining 1-3. Crude oil stimulates oxidative
stress in animals 4, 5. Lipid peroxidation, xanthine oxidase superoxide
dismutase (SOD) and catalase activities are part of oxidative stress indices
6. Lipid peroxidation elicits oxidative damage in plants and animals and its
value in conjunction with alterations in the level of antioxidants represent a
measure of oxidative stress. Similarly, the activity of xanthine oxidase is a
defense mechanism as well as measure of oxidative stress in animals 6. Report
has it that the deleterious action of crude oil on the kidney is based on
oxidative stress 7.

 Byproducts of the oil palm tree are important
medicinally. This is because the leaf juice have wound healing property while
the sap is used as laxative 8.This is due to 
 compounds rich in medicinal and antioxidant
properties inherent in oil palm leaf 9, 10. 
The antioxidant action is attributed to the presence of phytochemicals (flavonoid,
tannin and phenols) in the leaves of oil palm tree 11. In fact, oil palm leaf
extract contains more antioxidative phenolic compounds than various green tea
extracts 12. Therefore, oil palm leaf extract is a potential source of
functional food ingredient, based on reports of its health benefit 13 .This
study is aimed at evaluating the protective potentials of oil palm leaf against
crude oil contaminated diet induced nephrotoxicity in rats.

2.0 Materials
and methods

The crude oil used for this study was obtained from
Nigeria National Petroleum Corporation (NNPC) Warri, Delta State, Nigeria. The
palm leaf used was obtained from Elaeis guineensis tree in Obiaruku,
Delta state, Nigeria Forty eight (48) female albino wistar rats with weights
ranging from 0.088kg to 0.182 kg obtained from the animal house of Department
of Anatomy, Delta State University, Abraka, Nigeria were used for this study.
The rats were housed in a standard wooden cage made up of wire gauze, net and
solid woods and left to acclimatize for one week on grower’s marsh and tap
water at laboratory temperature of 28o C and
12 hour day/ night regime. After the acclimatization period, the rats
were weighed and grouped.

2.1 Preparation
of leaf powder.

leaves were isolated from the stock and sun- dried. The dried leaf was then
ground with domestic kitchen blender into a fine powder and stored in a clean
and sealed plastic container

2.2 Treatment
of animals

The forty eight (48) female albino wistar rats were
assigned to six (6) groups according to their weights, with eight rats in each
group. Rats in the control, Group 1 were fed with grower’s marsh only. Rats in
Group 2 were fed with grower’s marsh treated with 5g of powdered palm leaf.
Group 3 rats were fed with grower’s marsh treated 10g of powdered palm leaf.
Group 4 rats were fed with grower’s marsh contaminated with crude oil (4ml per
100g of feed).This concentration of crude oil in diet was
established to be tolerated by the rats over a long period in a preliminary
study. Rats in Group 5 were fed grower’s marsh contaminated with crude
oil (4ml per 100g of feed) plus 5g of powdered palm fronds. While rats in Group
6 were fed with crude oil contaminated marsh (4ml per 100g of feed) plus 10g of
powdered palm leaves. The rats in each group were allowed access to clean
drinking water while the experiment lasted. The feeds were prepared fresh daily
and stale feed remnants were discarded regularly. This
was done every morning between the hours of 8 am – 9 am and each group provided
with 400 g of the respective diet. The animals in each group were
exposed to their respective diets for three and six months respectively. The National Institute of
health guide for the care and use of laboratory animals (NIH, 1985) was adhered
to in the course of the experiment



2.3 Collection
of samples

After three months, four rats were sacrificed in each
group and the kidneys collected. Five grams (5.0 g) of the kidneys were weighed
in chilled conditions and homogenized with 5ml of normal saline in a mortar. The mixture was diluted with 45 ml of buffered saline (pH
7.4) before it was subjected to centrifugation at 2, 500 rpm and the
supernatant was transferred into plastic tubes and stored at – 4o C in the refrigerator before
used for analysis within forty eight hours. This same procedure was adopted
after six months exposure period.

2.4 Determination of lipid peroxidation and xanthine oxidase activity

The activity of
xanthine oxidase in the kidney of rats was measured using the method of
Bergmeyer et. al. 14, a reaction based on the oxidation of xanthine to uric
acid, a molecule that absorbs light maximally at 290 nm. A unit of activity is
that forming one micromole of uric acid per minute at 25oC. Lipid
peroxidation in the kidney of rats was measured by the thiobarbituric acid
reacting substances TBARS, method of Gutteridge and Wilkins 15.Total
superoxide dismutase activity was assayed using the method of Misra and
Fredorich 16. Catalase was assayed as reported by Rani et al. 17  

2.5Statistical Analysis

of variance (ANOVA) and post Hoc Fisher’s test for multiple comparison
was performed using statistical package for social science (SPSS), version 20  to determine statistical significant
differences between means. P values


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